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Moderate-to-vigorous cardio sessions lasting 30 to 40 minutes elevate circulating BDNF by roughly 30 percent, and these elevated levels persist for up to 24 hours

70 mL of 10 % HCl was added to the resulting concentrated solution which was then heated and stirred at a temperature of 80 to 90 0 C for 2 hours, concentrated by distillation under reduced pressure, and recrystallized from small amounts of anhydrous ethanol and acetone to afford L-carnitine hydrochloride

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