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After stirring 1 h, the solvent was removed under reduced pressure, the organic layer was extracted with ethyl acetate (15 ml), and then washed with 2 5 ml of 10% citric acid, 2 5 ml NaHCO3 (1M), and then with 2 5 ml brine
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Analysis was performed using a human proteome database from December 2022 (Uniprot) with contaminants added and with Streptavidin added manually as a contaminant
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