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Generally, wait at least a few days after invasive procedures before resuming GHK-Cu

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Add the reconstitution volume slowly along the vial wall rather than directing the stream onto the lyophilised cake, swirl gently until fully dissolved, and dont vortex as mechanical agitation can cause peptide and protein aggregation

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The vector was first digested with BsmBI (R0739L, NEB, USA) and dephosphorylated with CIP (M0525S, NEB) to facilitate the insertion of the SYNCRIP target sequence