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96 YuanH.LiX.ZhangX.KangR.TangD
Topical for convenience and localized facial concerns
LCMS/MS analysis was performed using an Advance nanoLC (BrukerMichrom, Auburn, CA, USA) and LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific) equipped with a NANOHPLC capillary column C18 (0.075 mm ID 150 mm length, 3 m particle size, Nikkyo Technos, Tokyo, Japan) using a linear gradient (25 min, 535% CH 3 CN /0.1% formic acid) at a flow rate of 300 nL/min
This kind of trial is expensive typically several million dollars and to date has been more difficult to justify economically because GHK-Cu cannot be patented as a novel drug (the molecule is endogenous and the relevant patents have expired)
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