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As the extent of their methylation was much lower in the brain and in lower passage astrocytes, the authors concluded that their high methylation may just be an in vitro artifact of the prolonged passaging of cells (Meissner et al., 2008), a phenomenon that was previously observed (Shen et al., 2006

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Study design, inclusion criteria, endpoints, comparator groups, sponsor involvement, duration, and monitoring procedures all affect interpretation

(2009) Efficacy of HGF gene transfer for various nervous injuries and disorders