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All experiments with sygl-1(ubiq) and lst-1(ubiq) were carried out at 15C to maximize tumor penetrance, except those in S4A and S4B Fig , where tumor penetrance was tested with different temperature regimens, and in S5ES5J Fig , where epistasis with fbf-1 fbf-2 was assayed at 25C

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Specifically, treatment with Nano-Lut restored pain threshold latency and significantly increased square crossings and exploratory behavior in the OFT, indicating recovery of animal behavior after treatment