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This does not reduce the total amount of GHK-Cu delivered

Results can be positive, negative, or inconclusive, and a positive result may be confirmed with a second lab test to avoid penalties from false positives

They are also involved in the process called apoptosis, a deliberate removal of cells

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The cells were collected 48 h post-transfection, washed twice with PBS and lysed in 1 ml of HEPES buffer (20 mM HEPES-KOH (pH 7.9), 300 mM KCl, 0.2 % NP-40, 15 % glycerol, 1 mM DTT and protease inhibitors, Sigma-Aldrich, P8340) for 20 min