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Yet eight years later, at the outset of his Ph.D., Sikiric managed to inspire a small band of colleagues to join him in the search for this hypothetical compound, which he dubbed Substancija Boze Pomozi, or, roughly, substance God help me. First, they needed gastric juice

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InStatPearls [Internet] 2022 Jul 4

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(6)Fmoc-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (6) Preparation of Fmoc-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (5)Fmoc-Asp(otBu)-Ala-Gly-Leu-Val-WangResin(20DMF)5203DMF3DMF2Fmoc-Asp(otBu)-OH1.1gDIEA0.4mlTBTU0.56gHOBT0.2gDMF10ml1.53DMF3DMF2Fmoc-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin In the Fmoc-Asp(otBu)-Ala-Gly-Leu-Val-WangResin in step (5), add decapping reagent (20% piperidine in DMF solution), react at room temperature for 5 minutes, drain, and add decapping reagent at normal temperature React for 20 minutes, wash with isopropanol for 3 times, DMF for 3 times, DMF for redistilled for 2 times, drain, add Fmoc-Asp(otBu)-OH1.1g, DIEA0.4ml, TBTU0.56g, HOBT0 .2g, DMF10ml normal temperature reaction for 1.5 hours, drained with a vacuum pump, washed 3 times with isopropanol, washed 3 times with DMF, washed 2 times with DMF evaporated, and dried to obtain Fmoc-Asp(otBu)-Asp(otBu )-Ala-Gly-Leu-Val-WangResin

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