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The membrane was then incubated at room temperature in blocking solution for 1 hour, followed by incubation in blocking solution containing the appropriate dilution of either phospho-Jak2 or the primary antibody for growth hormone receptor, for 2 hours

After discarding the protein solution, blocking buffer (1% Casein/TBS-T) was added and incubated for 2 h at room temperature

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The stimulated superfusion medium was similar to the basal one with the exception of GSH or L-aspartate added as a stimulus for GABA release

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