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Because existing uEV isolation methods often suffer from limited scalability, contamination by abundant urinary proteins, and bias toward specific EV subpopulations, the authors benchmarked the MSP approach against differential ultracentrifugation (dUC) and size-exclusion chromatography (SEC)

The robustness and effectiveness of functional annotation of BioFung compared to similar tools have been explored in Supplementary Figure 1df, demonstrating the utility of this tool

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Furin also regulates the transcription factor NKX2-5, which is essential for both normal heart development and CV function 346