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ROS are generated by the NADPH oxidase (NOX) enzymes, located on the plasma membrane of cells, notably in neutrophils and vascular endothelial cells

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10 Cysteine and methionine Cysteine is a sulfur-containing amino acid that can be either obtained from dietary sources or synthesized via the transsulfuration pathway

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Preclinical studies (Iselin et al.,1988, and Curr & Preziosi, 1997) confirm that nicotine-induced relaxation of gut muscle depends on intact VIP signaling, reinforcing this connection