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Real-time PCR was performed using LightCycler 480II (Roche, Basel, Switzerland)

SILAC proves optimal for investigating oxidative stress mechanisms in controlled cell culture models, while TMT or LFQ may be preferred for larger patient cohorts or clinical samples, considering their respective strengths in quantifying redox-related proteins in CF

Any doses cited describe clinical-trial protocols or approved labels for context only, and research-market material is of unverified identity and purity

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"A genome annotation-driven approach to cloning the human ORFeome"