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Graphical Abstract Similar content being viewed by others Abbreviations DLAT : Dihydrolipoamide S-acetyltransferase FDX1: Ferredoxin 1 GCLC: Glutamate-cysteine ligase catalytic subunit ICB: Immune checkpoint blockade ICD: Immunogenic cell death L-BSO: L-Buthionine-(S,R)-sulfoximine LIAS: Lipoic acid synthetase SLC31A1: Solute carrier family 31 TIME: Tumor immune microenvironment TNBC: Triple-negative breast cancer Funding This work was supported by the National Key Clinical Specialty Construction Program, the Science and Technology Program of Chengdu (2024-YF05-00487-SN), the Science and Technology Program of Tibet Autonomous Region (XZ202401RK0005), the Sichuan Science and Technology program (2023YFS0133), the Natural Science Foundation of Xiamen Municipality (3502Z202374035)

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Amplification products were size selected and purified with Agencourt AMPure XP system (Beckman Coulter) following manufacturers instructions, and subjected to bidirectional Sanger sequencing with second round amplification primers

[3] These complexes have three central subunits: E1-3, which are the decarboxylase, lipoyl transferase, and dihydrolipoamide dehydrogenase, respectively

Many treatment protocols include Vitamin C for additional antioxidant support