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Embryos were deteriorated by forceps, anesthetized in tricaine methanesulfonate solution (Sigma-Aldrich), mounted in 1% methyl cellulose on a depression slide (Aquatic Eco-Systems, Apopka, FL, USA) and photographed under the stereomicroscope Z16 (Leica Microsystems, Ernst-Leitz-Strasse, Germany) for observation

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This method of administration bypasses the digestive system, which can degrade glutathione, ensuring maximal absorption and utilization by the body's cells

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