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10% fetal bovine serum/FBS (Life Technologies 10270106) and 1% penicillin/streptomycin solution (P/S, Sigma-Aldrich P0781) were used for cell culture, except for DHODH knockout cells, which were cultured with the additional supplement of 50 M uridine at 37 C in a humidified incubator containing 5% CO 2

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Often the dramatic weight loss from calorie restriction is a result of loss of both fat and muscle

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(2) the branched-chain -ketoacid dehydrogenase complex