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J Biol Chem (2013) 288(35):2508897.10.1074/jbc.M113.452516 194 EverardALazarevicVDerrienMGirardMMuccioliGGNeyrinckAMet alResponses of gut microbiota and glucose and lipid metabolism to prebiotics in genetic obese and diet-induced leptin-resistant mice

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The pH of each sample was adjusted within the range of 7 to 9 using 014 pH strips (#13640516, Thermo Fisher Scientific) before subjecting them to digestion with mass spectrometry-graded trypsin (#90057, Thermo Fisher Scientific) at a 1:20 trypsin to protein (w/w) ratio