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Through its conversion to ornithine and proline, arginine directly supplies the substrates required for collagen biosynthesis, positioning it as a metabolic driver of ECM accumulation [13,14,15,16]

In a microwell ELISA plate pre-coated with anti-mouse glutathione peroxidase (GSH-Px) antibody (solid phase antibody), add mouse glutathione peroxidase (GSH-Px) calibrator and the sample to be tested, then add biotin-labeled antibody, incubate and fully wash, then add HRP-coupled avidin, incubate and fully wash to remove unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid surface of the microwell plate

FOXO4-DRI, designed to disrupt the FOXO4-p53 interaction, was tested on senescent TM3 Leydig cells

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