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After 36 h coincubation, cells were washed with cold PBS and evaluated for luciferase luminescence according to the manufacturers instruction (#RG051S, Beyotime)

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Tumor cells with elevated intracellular GSH levels often induce overexpression of GGT

This reversal was seen also on the background of the disturbed prostaglandins- and NO-systems, prostaglandins synthesis inhibition as well as NO-overstimulation (NOS-substrate L-arginine) or NO-blockade (N(G)-nitro-L-arginine methyl ester (L-NAME)) (Stupnisek et al., 2012