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GHK-Cu stands for Glycyl-L-Histidyl-L-Lysine-Copper, a naturally occurring copper peptide found in human plasma, saliva, and urine

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The cells were incubated at room temperature for 30 minutes in the dark, and the cell cycle was analyzed by flow cytometry (Win Bryte HS cytometer [Bio-Rad], using software Win Bryte, Bio-Rad Laboratories Inc., Hercules, CA, USA)

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This questionnaire has not undergone validation process was specifically designed for the study, based on the most common and debilitating symptoms of hyperthyroidism, which often drive the clinician to use additional drugs