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Differential gene expression analyses were performed using nSolver, which applies several multivariate linear regression models to identify significant genes (mixture negative binomial, simplified negative binomial, or log-linear model)

For the cerebrovascular uptake study ( n = 22), male and female homozygous APOE2-, APOE3-, and APOE4-TR mice were euthanized at 26-weeks (Supplementary Table S2)

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