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Testis were homogenized in 50 mM NaCl, 1 mM EDTA, 1% Triton-X-, and 20-mM TRIS-HCl pH 7.4, by using a IKA Ultra-Turrax homogenizer

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The frequency of subclinical hypothyroidism in obese children and adolescents and its relationship with metabolic parameters and atherogenic index