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After lysis and centrifugation, the proteins were isolated by affinity chromatography with Ni 2+ -NTA agarose resin and the 6xHis tag was cleaved using TEV protease except for UBA1

The levels of adenine nucleotides and pyridine coenzymes in red blood cells from the newborn, determined simultaneously by HPLC

however, only astrocytes could activate the adenosine monophosphate-activated protein kinase and therefore compensate for the diminished ATP levels by upregulating glycolysis