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The Cobas z 480 instrument (Hoffmann-La Roche Ltd., Basel, Switzerland) was used to perform the qPCR according to the following protocol: 2 min at 50 C, 10 min at 95 C, 45 cycles of 15 s at 95 C, and 1 min at 60 C

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[Google Scholar] Ferrari R., Merli E., Cicchitelli G., Mele D., Fucili A., Ceconi C