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doi: 10.1080/00498250110079149

and Mollin, D

Product Features Additional Notes Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data Standard Curves Spiking & Recovery Results Linearity Results Application Notes Kit Components Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate Other Materials Required Distilled or deionized water Precision pipettes to deliver 2 l to 1 l volumes Adjustable 1-25 l pipettes for reagent preparation 100 l and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis Protocol Outline Prepare all reagents, samples and standards as instructed in the manual

Body weights, food intake, and body composition were measured throughout the study