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HeLa cells were co-transfected with pX458-gRNAs and the donor plasmid via Lipo3000, followed with G418 selection (800 g/mL)

ROS-induced endothelial dysfunction in the pathogenesis of atherosclerosis

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According to the several studies conducted with different instrumental techniques, such as by Nuclear Magnetic Resonance ( 113 Cd-NMR [152]), X-ray diffraction, and molecular mechanics and dynamics calculations [153], metallated metallothioneins look like protein chains wrapped around a metal cluster, with multiple cysteines liganding metals through their thiolate groups [154], as exemplified in Scheme 13 for the tetra-metallated domain of a Cd-loaded MT