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Additionally, molecular docking, in conjunction with drug affinity responsive target stability assay (DARTS) and cellular thermal shift assay (CETSA), was conducted to evaluate the possible target proteins for kellerin
Nature 586 , 583588 (2020)
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For the generation of Go-Nt 7 -FM 4 -GFP and MGNC-Nt 7 -FM 4 -GFP, the FM 4 sequence was PCR-amplified from the GFP-FM 4 -hGH plasmid (Andrew Peden, University of Sheffield), cut with AgeI/PstI and ligated in frame into the same sites of Go-Nt 7 -GFP and MGNC-Nt 7 -FM 4 -GFP
If you are working with lyophilized tirzepatide powder, you decide how much bacteriostatic water to add
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