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The barrier tightness of the model was verified by transendothelial electrical resistance (TEER) measurements using an EVOM voltohmmeter (World Precision Instruments, USA) combined with STX-2 electrodes

GSSG in the samples was reduced with tris(2-carboxyethyl)phosphine (TCP) to GSH, and then derivatized with ABD-F to obtain the total concentration of thiols ([GSSG] = [GSH] total [GSH, prior to reduction with TCP])

Certainly, we will study all about them

Acknowledgements We thank Dr Serge Luquet, Giuseppe Gangarossa, Nicolas Thiebaud and Claire Martin for their advice and helpful discussions