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GF mice are devoid of any microbiota, and are an efficient model to understand the functional significance of compositional changes in the gut microbiota: fecal microbiota transplant from metformin-treated individuals to GF mice were able to transfer the improved glucose tolerance phenotype to the recipients (45)

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HaCaT cells were subsequently engineered to express the HPV-16 E7 Mut molecule (Figure S1), in order to evaluate its effects in various biological assays when compared to the wild-type molecule