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To monitor the transfer of the glutathione moiety from PfGrx to roGFP2, 5 M reduced roGFP2 WT , roGFP2 C151S or roGFP2 C208S were mixed with variable concentrations of glutathionylated PfGrx C88S or PfGrx C32S/C88S at room temperature for 1 h