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Data availability The data analysed in the current study are available from the corresponding author on reasonable request

Adults need around 2.4 micrograms (mcg) of vitamin B12 a day

Clinical trials confirmed no effect on serum IGF-1 levels, no negative effect on carbohydrate metabolism, and no anti-AOD-9604 antibodies detected in any participants

B12 shots skip the digestive system as they enter the bloodstream directly

Additional preclinical work published in Cell Chemical Biology evaluated NNMT inhibition in similar diet-induced obesity models and confirmed the pattern: significant reductions in body weight gain, adipose tissue expansion, and circulating triglycerides compared to untreated controls