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Mitochondrial respiratory complex activity measurement The enzymatic activities of complex I (NADH ubiquinone reductase), complex II (succinate ubiquinone reductase), complex III (ubiquinol cytochrome C reductase), and complex IV (cytochrome C oxidase) were measured in macrophages using enzyme activity assay kits (A089-1-A089-4, Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturer's instructions
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Clinical indications include deficiency due to inadequate diet or malabsorption, drug-induced deficiency (for example, from long-term isoniazid or oral contraceptives), and certain inborn errors of metabolism (such as pyridoxine-dependent seizures or sideroblastic anemia)