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Because vitamin B12 has a wide array of cellular actions the symptoms of deficiency are quite diverse

We first determined whether ML226 and DEG had similar effects on 2-OGDDs and examined PHD activity (HIF-1 stabilization), TET activity (DNA 5-hydroxymethylcytosine (5hmC) levels) and KDM activity (histone lysine methylation marks)

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The study quantified oxidative and nitrosative stress markers, mitochondrial respiratory chain complex activities, and inflammatory mediators such as TNF- and MMP-9, using spectrophotometric assays and ELISA