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GSH concentration is instead measured by either of two methods: by analysis of GS-NEM conjugates by HPLC in the same sample that is used to measure GSSG or, alternatively, by analysis of GSH by spectrophotometry (GSH recycling method) on one additional sample aliquot that has not been derivatized with NEM
Our bodies don't store it in large amounts, making regular intake essential
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Circular dichroism Peptides at concentration of 1 mg/ml, diluted in 10 mM HEPES pH 7.0 or PBS, without or with 2,2,2-trifluoroethanol (TFE, Sigma-Aldrich, St Louis, MO, United States) at concentrations in the range of 525% (commonly used to stabilize secondary structure of polypeptides), and diluted in 10 mM HEPES pH 7.0 with 30 mM SDS (BioShop Canada Inc., Burlington, Canada) (to mimic anionic environment) were prepared