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2.7 RNA extraction and qRT-PCR Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out according to our previous published protocol ( GLP-1 and MPC2 expression levels were normalized to GAPDH and calculated via the 2 CT method

Ketogenic or very-low-carbohydrate diets can be a practical approach to weight loss and glycemic control for some people, while others find long-term adherence difficult [[284], [285], [286]]

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