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Alexa-Fluor 594-conjugated donkey anti-mouse IgG was diluted 1:500 to 4 g/ml (Invitrogen, A21203) as secondary antibody and applied on sections for 1 h at room temperature

Insulinotropin: glucagon-like peptide 1 (737) co-encoded in the glucagon gene is a potent stimulator of insulin release in the perfused rat pancreas

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Further, clinical trial studies of MK-677 have been conducted to improve body composition and metabolism in older adults11 or to increase bone mass in obese young males and older adults.12, 13 However, the beneficial effect of MK-677 on growth promotion in children is controversial