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Oil red O staining Organoids were fixed in 4% paraformaldehyde (Electron Microscopy Services), cryoprotected in 30% sucrose solution overnight, and embedded in optimum cutting temperature (OCT) compound (Tissue Tek)

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In physiological conditions, the maintenance of normal glucose metabolism depends on the interaction of insulin, glucagon, islet amyloid, and incretin (Aronoff et al

Table 2 provides examples of the various fabrication methods discussed