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The FDA recommends that HF validation tests be conducted before major clinical trials so that the HF-validated device can be used in the clinical trials to achieve the most accurate results possible

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doi: 10.1523/JNEUROSCI.3306-14.2015 85 GarretsonJTSzymanskiLASchwartzGJXueBRyuVBartnessTJ

The absorbance for each condition was measured, and the percentage of cross-reactivity was calculated using the following equation, adapted for non-competitive sandwich immunoassays 53 : Cross-reactivity (%) = [(OD of cross-reactant analyte)/(OD of target analyte)] 100