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Disrupting melanosome transport may also induce autophagy-mediated degradation of accumulating melanosomes, which can contribute to the decrease of melanin content in cells [126]

In brief, after labeling and washing, cells were lysed with radioimmunoprecipitation assay (RIPA) lysis buffer supplemented with 1 protease inhibitor cocktail (Thermo Scientific, 78429)

One significant contribution was made by Hyman Zimmerman in the 1960s, who observed that patients exhibiting hepatocellular DILI alongside jaundice had a notably elevated risk (between 10 and 50%) of progressing to acute liver failureduring a time when liver transplantation was not yet an option

This review systematically summarized current knowledge on TCM-derived compounds targeting NLRP3-mediated pathways in AP, while critically addressing persisting challenges in translational applications

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