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Excess DTT and imidazole were removed on a PD-10 desalting column (Merck), and the reduced proteins were eluted with 3.5 mL ice-cold assay buffer containing 100 mM Tris/HCl, 1 mM EDTA, pH 8.0

Additionally, maintaining a diet rich in sulfur-containing foodssuch as broccoli, cauliflower, Brussels sprouts, and garlicprovides natural dietary support that complements the NAC in the formula, ensuring your body has a constant supply of the elemental building blocks needed for endogenous antioxidant synthesis

ThandivelSRajanPGunasekarTArjunanAKhuteSKaretiSRet al

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10.1002/2211-5463.12402 103 WanbiaoZ.JingM.ShiZ.TengxiangC.XuekeZ.HaiyangL