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The main biochemical characteristic of ferroptosis is iron-dependent over-oxidation of polyunsaturated fatty acids (PUFAs)-containing phospholipids (PLs) on cell membranes ( RPL8 ), iron response element binding protein 2 ( IREB2 ), ATP synthase F0 complex subunit C3 ( ATP5G3 ), citrate synthase ( CS ), tetratricopeptide repeat domain 35 ( TTC35 ) and acyl-CoA synthetase family member 2 ( ACSF2 )

The conserved lysine residue N-terminal to the active site, Cys A , helix 1 and helix 3 as well as the -sheets of the thioredoxin fold are almost perfectly superimposed