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The mobile phase was acetate buffer [0.25 mol/L ammonium acetate solution-0.1 mol/L ethylenediaminetetraacetic acid disodium solution-triethylamine (100:10:1), adjusted to pH 8.8 with glacial acetic acid or ammonia solution]-acetonitrile (85:15, v/v) with a flowrate of 1.0 mL/min