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[DOI] [PubMed] [Google Scholar] 102.Saheed S., Frans Hendrik O., Tom A.A
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Role of GST P1-1 in mediating the effect of etoposide on human neuroblastoma cell line Sh-Sy5y
Primers were designed to fit in resulting vector, pJB-HTS upstream of the hexa-histadine tag (5/phos/CCATGGGCAGCAGCCATCATCAT), and downstream (AGCTGGAATTCCTAGTTATTGCTCAGCGGTGGC) (Integrated DNA technologies) of the spacer yielding a fragment containing a phosphorylated 5 end, an initiation codon, a hexa-histadine tag, a thrombin cleavable sequence, a spacer, a termination codon, and an EcoRI site
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