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Using single particle tracking (SPT), it was shown that TGEV moves more freely in 3-day mucus as compared to 3-week mucus measured in terms of diffusion coefficient calculated by means of multiple trajectories over a short period of 5 s [109]
F.RasmussenS.RossingP.IdornT
After three weeks of virus expression, immunofluorescence staining confirmed that EGFP + signal largely co-localized with signal from glutamate-specific antibody in PVN, indicating specific targeting of PVN Glu neurons (Fig
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