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After washing the slices with PBS three times, we incubated them with Alexa Fluor 647 goat anti-rabbit secondary antibody (A-21245, Thermo Fisher Scientific, Waltham, MA, USA), diluted 1:500 in the blocking solution, for 2 hours at room temperature

Readers with existing health conditions, those taking prescription medications, and those who are pregnant or breastfeeding should consult a licensed healthcare professional before use

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The blood vessels also become more fragile

Prior authorization for Wegovy typically requires documented obesity (BMI 30) or overweight with comorbidity, plus evidence of previous lifestyle intervention attempts