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Luteolin alleviated HPVR via the HIF-2-Arg-NO axis and the PI3K-AKT-eNOS-NO signaling pathway, protecting PAECs function, with efficacy comparable to that of sildenafil

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Aggregation kinetics were monitored in sterile black 96-well plates with clear bottoms (Corning Incorporated Costar Scientific) at a final reaction volume of 100 L per well using an Infinite M200 PRO multimode plate reader (TECAN, Switzerland)