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For example, the intrinsically disordered C-terminal domain of chicken gizzard caldesmon (CD136) has a max of 350.2 nm, but interaction with its binding partner, calmodulin, results in a pronounced (17 nm) blue shift of the CaD136 fluorescence spectrum (Permyakov et al., 2003)

Bendotti et al

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The observed phase transition temperatures were indeed independent, within the experimental uncertainty, of the laser pulse power and duration, whereas, of course, longer and more powerful laser pulses implied higher peak temperature, longer dwelling time beyond melting, larger vapour production etc