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We refer to this as die off

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2.9 Protein expression and purification The gpoA gene from the pneumococcal R6 strain was cloned into the pET15b expression vector and transformed into Escherichia coli BL21 (DE3) cells (Thermo Fisher Scientist)

We assume that both treatments, notably the niosomal forms, significantly succeeded in protecting against ATZ-induced steroidogenic machinery disruption through overexpression of 3-HSD , STAR , and CYP11A1 in the testes