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Although increasing attention has been given to the role of ferroptosis in OA to date, most researchers pay more attention to chondrocyte ferroptosis and cartilage degeneration rather than synovitis

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The in vitro experiments were performed using TM3 mouse Leydig cells that were cultured in DMEM/F-12 medium supplemented with 5% horse serum and 2.5% fetal bovine serum, and maintained at 37C under a 5% CO2 atmosphere

It was originally identified during research on natural sleep-promoting compounds produced by the body