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In this Perspective, we review methodological issues in sleep and memory studies and suggest constructive solutions to address them

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A pre-heparinized syringe was used to collect 300 L of blood sample from the lateral tail vein on day 1 at 0 (pre-dose), 0.08, 0.25, 0.5, 1, 2, 4, 8, 24, 48, and 72 h and on day 7 at 0 (pre-dose), 0.08, 0.25, 0.5, 1, 2, 4, 8, 24, 48, as well as 72 h after test substance administration

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It was shown through electrophysiological recordings that the reduced expression of L-type and P/Q-type Ca 2+ channels was responsible for reduced transmembrane Ca 2+ currents and decreased insulin granule exocytosis, where the reduction of L-type channels mediated the majority of the effect