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Histological examination After being separated from eyeballs preserved in 10% w/v formalin, the cornea, retinal, and scleral tissues were processed and embedded in blocks of paraffin wax

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By degrading GSH, CHAC1 perturbs redox homeostasis and can effectively blunt the NRF2-driven cytoprotective response

To obtain the specific labeling patterns, intracellular metabolites were extracted and analyzed using GC/MS to determine multiple ion detections (MIDs) (corrected for natural isotope abundance), which reflect relative metabolic fluxes 28